The manual processing of samples in many laboratories often takes up a great deal of the laboratory staff’s time. This is true both for solid phase extraction and for subsequent concentration (evaporation). Reproducibility is impaired by the many manual steps, such as the manual rinsing of the loading containers or the use of a rotary evaporator. This can lead to inaccurate or inconsistent results and undermine trust in the methods. Automation of these processes can provide assistance here and helps to improve the efficiency and reproducibility of the methods.
The column is firmly connected to the arm, therefore sample and solvent can be dosed precisely and at a constant flow rate via the SPE column with positive pressure using a syringe pump. Drying is also carried out via positive pressure with nitrogen.
Double-walled with two independent lines inside the needle. Spray holes are arranged 360° around the needle:
More than 40 different racks (also temperature-controlled) can be freely combined with the FREESTYLE system.
Maximum flexibility for your applications, just as you need it, also for IAC columns. Only one rack type is needed for all columns in standard formats up to 15 mL. Adaptation is done by adapters. The racks are hooked in easily and transferred to the software with just a few mouse clicks.
A direct, automated elution of the SPE column, directly into the EVAporation chamber within one method (online), is particularly convenient. An optional liquid/liquid solvent exchange completes the automation.
For more details on the advantages of the EVA module, please refer to the corresponding product page.FREESTYLE EVAporation
QuEChERS à la LCTech is based on a non-dispersive clean-up step and has specially adapted columns. This covers almost all matrices within pesticide analysis in food and feed. The non-dispersive application with the columns saves time (no shaking, no centrifugation) and leads to better chromatography due to the high clean-up and the reduced matrix ion suppression associated with this. This is reflected in very good recovery rates with high reproducibility.FREESTYLE Pesticides
By modifying the FREESTYLE software, two solid phase extractions (“Dual-SPE”) with different column formats can be processed automatically one after the other. Specially developed plungers minimise the dead volume, increase flow rates and thus reduce the processing time per sample.
In combination with the EVAporations module, the FREESTYLE SPE takes over the complete clean-up for the analysis of petroleum hydrocarbons: Extraction, concentration to 1 mL and filling of the finished sample into a GC vial. Of course, the processes can also be carried out separately. For the clean-up step, we recommend the cost-effective Florisil Elufix™ columns.Elufix™ columns
The sample loading procedure for bidirectional SPE (solid phase extraction) enables even difficult matrices, such as brain tissue or similar matrices, to be processed automatically without supervision 24 hours a day, 7 days a week. Cross-contamination can thus be eliminated due to the nature of the procedure.
Urine samples from racehorses/camels are particularly difficult to process due to their viscosity, as this may cause a blockage in a column. A pressure check alone is not enough in such cases. The FREESTYLE software has an excellent solution: if a column becomes blocked, the processing of this sample is terminated, the sample is marked in the sequence list and the next sample is processed! Such features realise end-to-end automation without downtime.
The various types of sample loading open up many different possible application: from large volumes of up to approx. 100 mL in mycotoxin analysis down to just a few µL, such as in forensics.
The FREESTYLE technology offers the right solution for any application, which can simply be selected in the software when setting up the method.
These samples (up to approx. 100 mL) are ideally aspirated into the sample loop (possibly several times) via the needle (first Z axis) and then released onto the SPE column (second Z axis).
The column is conditioned and positioned in the wash port. The small sample volume (e.g. 500 µL) is directly pipetted into the column using the needle. The SPE unit then takes up the column again and the process continues.
Elution from the SPE column can be performed in many ways. Even highly complex elution processes can be carried out. The different procedures are saved as a macro in the software and can easily be integrated into a method. A selection of different racks enables elution into different containers, e.g. into a 2 mL or 5 mL graduated flask, if a defined final volume is needed.