Processing water samples to detect any harmful substances that they contain is an important task in official and commercial laboratories.
To detect a wide range of chemical compounds, large-volume water samples are usually processed and an extensive repertoire of individual steps is applied. The manual processing of these often time-consuming and monotonous steps poses a challenge for the laboratory staff in routine laboratories.
Parallelised automation, which processes all the necessary steps precisely, makes it much easier to process large-volume water samples in the laboratory, while also reducing variance in the results.
The column is firmly connected to the arm, therefore sample and solvent can be dosed precisely and at a constant flow rate via the SPE column with positive pressure using a syringe pump. Drying is also carried out via positive pressure with nitrogen.
In the processing station on the FREESTYLE platform, 3 SPE columns are processed simultaneously in 2 blocks: In block 1, 3 columns are conditioned, loaded and washed in parallel, while in the following block 2, 3 columns are dried in parallel.
The following videos show the processing station of the FREESTYLE XANA. They are identical for all XANA models:
The external processing station is responsible for the fluidics management of the water module with the pumping mechanics of up to 10 L samples, the valves and the sensor technology for process monitoring.
The sample rack offers space for 24 x 1 L sample bottles; optionally 3 sample containers with up to 10 L volume each can be connected to the system.
Automatic rinsing of the sample bottles possible for optimal quantitative transfer of the sample.
Maximum flexibility for your applications. Just as you need it.
Only one rack type is needed for all standard column (also IAC columns) between 1 and 15 mL, the adaptation of which is done by adapters. The racks are hooked in with a flick of the wrist and registered in the software with a few mouse clicks.
It is particularly convenient when the sample is eluted directly into the EVAporation chamber. The concentration is similar to that of a rotary evaporator, using heat and vacuum. A final volume between 0.2 mL and 5.0 mL can be freely adjusted and is achieved reproducibly. The possibility of a liquid/liquid solvent exchange completes the full automation.
For more details on the advantages of the EVA module, please refer to the corresponding product page.FREESTYLE EVAporation
1. SPE gripper places up to 3 columns from the SPE rack into block 1 of the processing station:
2. Conditioning, loading and rinsing of these 3 columns at the same time
3. The SPE gripper places the 3 processed columns from block 1 into block 2 of the processing station. SPE gripper takes the next columns (up to 3) from the SPE rack and places them into block 1 of the processing station.
4. Block 1: Conditioning, loading and rinsing of up to 3 columns at the same time. Block 2: Drying of the first 3 columns
5. SPE gripper places the 3 dried columns from block 2 back onto the SPE rack. The SPE gripper places 3 loaded and rinsed columns from block 1 into block 2 of the processing station. SPE gripper takes the next columns (up to 3) from the SPE rack and places them into block 1 of the processing station.
6. Block 1: Conditioning, loading and rinsing of up to 3 columns at the same time. Block 2: Drying of up to 3 columns at the same time.
7. The first 3 columns are eluted sequentially in vials or in the vacuum chamber of the EVAporation module. Filling into GC vials takes place following EVAporation to the desired final volume; the water sample is thus fully prepared for the subsequent analysis.