Mycotoxins are harmful to health and are present around the world. In order to be able to put food or feed into circulation safely, defined limits must be constantly monitored, which requires a reliable analysis as well as reliable sample preparation. Many foods and feeds are contaminated with more than just one mycotoxin, which makes a multi-mycotoxin screening necessary. A simple, fast procedure can be carried out reproducibly and reliably using automated sample processing: matrix reduction, thanks to the CrossTOX technology, is achieved through non-dispersive SPE, whereby effective clean-up is guaranteed and can be carried out around the clock.
The FREESTYLE for CrossTOX® offers a quickly automated, reproducible and robust method for sample preparation for multi-mycotoxin analysis, which can run without supervision around the clock and thus makes everyday work much easier. The FREESTYLE system offers two possibilities for the automated processing of CrossTOX® columns:
The CrossTOX® column enables the optimal separation of matrix interferences and interfering substances due to its non-dispersive SPE material in order to pass the analytes on to be measured via LC-MS/MS.
Aflatoxins B1, B2, G1, G2, sterigmatocystin, ochratoxin A, fumonisin B1 and B2, deoxynivalenol, nivalenol, 15-acetyl DON, 3-acetyl DON, DON-3-GLC, zearalenone, T-2 and HT-2, citrinin, diacetoxyscirpenol
The column is firmly connected to the arm, therefore sample and solvent can be dosed precisely and at a constant flow rate via the SPE column with positive pressure using a syringe pump. Drying is also carried out via positive pressure with nitrogen.
Fluidics management is a central component of the robotic system, where precise dosing and control of fluids is essential:
Double-walled with two independent lines inside the needle. Spray holes are arranged 360° around the needle:
More than 40 different racks (also temperature-controlled) can be freely combined with the FREESTYLE system.
Maximum flexibility for your applications, just as you need it, also for IAC columns. Only one rack type is needed for all columns in standard formats up to 15 mL. Adaptation is done by adapters. The racks are hooked in easily and transferred to the software with just a few mouse clicks.
The HPLC Direct Injection module is part of the FREESTYLE automated sample preparation system. It allows complete automation from crude extract to chromatogram without any manual intermediate step with excellent results at the same time, by means of online injection into any LC system. Finished samples can be injected into the HPLC by means of sample loop overfilling, whereby the injection volume can be parameterised.
Detailed and varied method creation and easy handling in routine are a successful balancing act in the FREESTYLE software.
Get to know the quality of sample clean-up with the CrossTOX® column for mycotoxin analysis. Sample clean-up is an important point in order to reduce matrix interference. In addition, the analysis device is kept at the sensitivity level required for optimal and reliable analysis.