The FREESTYLE ThermELUTE™ enables fully-automated sample preparation for the analysis of aflatoxins B1, B2, G1, G2 and M1 as well as ochratoxin A and zearalenone in all regulated matrices.
The FREESTYLE BASIC is equipped with the SPE and the ThermELUTE™ module for this purpose. The system is then combined with any HPLC system – the result is comprehensive automation with processing from the raw extract to the finished chromatogram without any manual working steps. Minimum workload for maximised sample throughput.
The extracted, diluted and filtered sample is loaded onto the high-performance SMART column via the FREESTYLE system. After washing, the column is heated, breaking the bond between the toxin and antibody (thermal denaturation). The toxins are now eluted in a large volume as aqueous eluate directly into the HPLC sample loop via partial filling. Via an interface, the sample is released to and taken over by the HPLC, derivatised (aflatoxin B1, G1) and analysed. Meanwhile, the next sample is already being prepared by the FREESTYLE.