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Aflatoxin B/G in Ramtilla (Guizotia Abyssinica)

Mycotoxins

Sample Preparation - Manual and Automated

Ramtilla (Guizotia Abyssinica)

Ramtilla is a composite plant of the family Asteraceae and is used in Ethiopia as a fodder plant. Through the massive occurence of aflatoxin B1 in June and November 2015 in milk products, it was discovered that this happened because of aflatoxin contaminated feed, which was caused by ramtilla and press cakes of seeds thereof. Consequently many shops changed from fresh milk to imported and powdered milk, so the capital city Adisabeba lost a significant amount of turnover in dairy sector (Food control 59; p773-779; 2016). 

Easy, Fast & Reliable

Automated Processing with FREESTYLE SPE

In almost every analytical laboratory, samples are routinely cleaned via SPE columns in order to obtain clean solutions for subsequent analysis or analyte concentration. The automation with FREESTYLE SPE is the perfect solution to simplify these routine working steps, to obtain the reproducibility of the results, and to receive good recoveries. 

Each manual SPE method which has already proven of value in the laboratory can be automated in a quick and easy manner. The application fields are wide: from mycotoxin and environmental analysis up to forensic applications and samples of doping control. 

Equip the racks with your samples, configure the required method in the easy to operate software and press the start button. From now on the processing is taken over by the FREESTYLE system.

Protocol of Manual Processing

Homogenise 10 g ramtilla (guizotia abyssinica) and add 1 g of sodium chloride. Extract the sample with 50 mL methanol/water (80/20 (v/v)) and 25 mL n-hexane to remove fat and oils. The extraction should be conducted for 20 minutes.

Filtrate the crude extract and dilute 2 mL with 12 mL PBS (contains 8 % tween 20). Load the sample onto the immunoaffinity column AflaCLEAN and wash the column with 10 mL water. 

Dry the column and elute the toxin with 2 mL methanol. Keep in mind that the column bed is incubated with methanol for at least 5 minutes in order to ensure the complete denaturation of the antibodies.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.

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