Sample Preparation - Manual and Automated
Goji-Berries and their Effect
Goji berries are member of the so-called „Superfoods“, since they contain many vitamins and minerals. Among other things the berries strengthen the immune system and regulate the blood pressure, so they are associated with many health issues. Goji berries are mostly offered as dryed fruit or component of breakfast cereal.
Immunoaffinity Columns OtaCLEAN for Ochratoxin A
Ochratoxin A is a naturally occuring mycotoxin, which is produced by Aspergillus and Penicillium species as primary contaminant in various food and feed stuffs, so in goji berries, too. For food and feed analysis and application clean-up purposes, LCTech developed the immunoaffinity columns OtaCLEAN. The columns guarantee best results even at difficult matrices.
Automated Processing with FREESTYLE SPE
The FREESTYLE robotic system with SPE module assumes routine laboratory tasks around the clock and even at weekends. The system processes unattended, yet reliably, various applications for mycotoxin analysis. All types of mycotoxin columns and SPE-standard formats can be automated on the FREESTYLE. The options for sample loading are as diverse as they are for elution. For convenience, even elution into a volumetric flask with 2 or 5 mL is possible, so that the eluted volume can be quickly adjusted to an exact value.
Protocol of Manual Processing
Homogenise 20 g of goji berries and add 2 g of sodium chloride. Extract the sample with 100 mL methanol/water (80/20 (v/v)) and add 50 mL n-hexane to remove fat and essential oils. The extraction should be conducted for 20 minutes in order to exclude extraction efficiencies.
Filtrate the crude extract and dilute 2 mL with 12 mL PBS (contains 8 % tween20). Load the sample onto the immunoaffinity column OtaCLEAN and wash the sample reservoir with 2 x 5 mL. Load the wash solution onto the OtaCLEAN column, too.
Elute the toxin with 2 mL methanol. Keep in mind that the column bed is incubated with methanol for at least 5 minutes to ensure the complete denaturation of the antibodies.