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Ochratoxin A in Rye Bread

Mycotoxins

Fully automated with FREESTYLE SPE

Sample Preparation and Analysis

Ochratoxin A is a naturally occurring mycotoxin, which is pruduced by Aspergillus and Penicillium species as primary contaminant in various food and feed stuffs. For food and feed analysis and application cleanup purposes, LCTech developed the immunoaffinity column OtaCLEAN. The columns guarantee best results even at difficult matrices. Particulary easily in combination with the FREESTYLE robotic system for fully automated sample preparation, day and night, including weekends.

Automated Processing with FREESTYLE SPE

Any manual SPE method that has proved successfully in your laboratory, can be automated without any problems. With FREESTYLE SPE you can process many different SPE-column formats from 1 to 15 mL. Such as the 3 mL OtaCLEAN immunoaffinity column of LCTech. Extract, filtrate and dilute the rye bread according to the description of the manual processing. Put your samples into the FREESTYLE SPE, equip the racks with OtaCLEAN columns, choose the method from the software and press the start button.

Especially fast...
        Especially simple...

  • Excellent recovery rates
  • No cross-contamination
  • Extremely fast and precise processing
  • Very simple and intuitive software

Protocol of Manual Processing

Homogenize 20 g of rye bread and add 2 g sodium chloride. Extract the sample material with 100 mL (methanol/water (80/20 (v/v))) and 50 mL n-hexane for at least 10 minutes. Filtrate the raw extract. You can centrifuge the extract. To facilitate the phase separation, dilute 10 mL with 40 mL PBS In case of precipitation or turbidity you can remove them by filtration.

Load 25 mL (represents 1 g matrix) onto a 3 mL OtaCLEAN column with a flowrate of max. 2 mL/min. Wash the sample reservoir with 10 mL de-ionized water and load this solution onto the immunoaffinity column. Dry the column by flushing air through it and eluate afterwards with 2 mL methanol. Take care, that the methanol incubates within the column bed for 5 minutes. Collect the eluate and dilute it to HPLC conditions of the subsequent analysis.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.  

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