In order to ensure that imported food meets our quality requirements, it has to be tested for impurities such as bacteria, pesticides, heavy materials and mycotoxins. The consumption of food contaminated with mycotoxins can be very harmful to health even in low concentrations, for example it can cause chronic diseases or even organ failure. Therefore, the legislation has set maximum permissible limit values for mycotoxins. If food exceeds these levels, it may not be imported. Recently, for example, high concentrations of aflatoxins B/G have been found in quinoa seeds.
Immunoaffinity Columns for Clean-up of Analytes in Food and Feed
LCTech offers a broad product range around sample preparation. It is our goal to facilitate your daily routine in the laboratory with our high quality semi- and fully-automated systems and devices as well as with our useful and cost effective consumables.
Immunoaffinity Columns for Clean-up of Aflatoxins B/G
The immunoaffinity columns AflaCLEAN, AflaCLEAN Select and AflaCLEAN SMART are suitable for sample preparation in routine analysis using HPLC with fluorescence detection or LC-MS. hey are designed for the purification of aflatoxins B/G in food and feed and achieve very good recovery rates in difficult matrices. The columns possess a very high matrix tolerance and are able to bind the aflatoxins highly specific. The columns are available in a convenient 3 mL polypropylene format or additionally as 1 mL version and can be processed either manual or automated e.g. with the robotic system FREESTYLE SPE.
For a faster processing of your samples LCTech offers SMART columns in a small 3 cm format. For example, emptying of the column is not necessary and sample loading and washing of the column only takes 4 minutes at the most with a 10 mL sample volume and a flow rate of 3 mL/min. Since a maximum of only 400 µL are used for elution, processing time will be reduced even further.
Protocol of Manual Processing
Homogenise 10 g of quinoa seeds and add 1 g sodium chloride, 100 mL 80/20 (methanol/water 80/20 (v/) and 50 mL n-hexane. The extraction should be performed for 10 - 15 minutes. Filtrate the extract and centrifuge it for the phase separation between the aqueous (lower) phase and the n-hexane (upper) phase with 2000 x g for 10 minutes. Dilute 10.5 mL of the aqueous (lower) phase with 64.5 mL PBS-buffer.
Load 25 mL extract onto the AflaCLEAN / AflaCLEAN Select immunoaffinity column. Afterwards wash the column with 10 mL deionised water and load this solution onto the IAC-column, too. Dry the column and elute the toxin with 2 mL methanol. Keep in mind, that the methanol incubates for 5 minutes into the column bed, in order to dissolve the antibody toxin bond completely.
Dilute and measure the eluate to HPLC conditions.