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Ochratoxin A in Flour

Mycotoxins

The Flour

Flour is primarily the fine powder obtained from the grinding of cereal grains. People used the ground grain to produce food more than 100,000 years ago. Flour serves as the basis for a wide variety of foods and is therefore essential, especially in the food industry.

The best-known types of flour include wheat, rye and spelt flour. These are mainly used in cooking. In addition to cereals, various seeds such as  quinoa or legumes like peas are also processed into edible flour. On the other hand, fish flour is used as an animal feed and bone flour as fertilizer. Incorrect storage conditons result in the formation of mycotoxins such as aflatoxins or ochratoxin A in grain, which can be the cause of poisoning. Since mycotoxins are highly harmful to human an animal health in excessive quantities, flours and grains are regularly tested for them.
 

Fast and Efficient Clean-up - Manual and Automated

Ochratoxin A is one of the most highly regulated mycotoxins and is formed by molds of the genus Aspergillus and Penicillium. Since mycotoxin clean-up is particularly important in the food and feed sector today, LCTech has developed a manual way to efficiently prepare samples with the OtaCLEAN immunoaffinity columns and an automated way with the FREESTYLE SPE robotic system.
 
More samples in less time and high cost savings. Very good recoveries are also achieved with matrices such as  flour. Any manual SPE method that has proven successful in your laboratory can be transferred directly to the robotic system. Already created methods can be saved and reused, but also modified.
 

Processing Protocol

Extract 10 g of flour through 50 mL methanol/water (80/20 (v/v)). Perform the extraction between 3 and 10 minutes to obtain high extraction efficiency.
Filtrate the raw extract and dilute 10 mL of it with 40 mL of PBS. Remove precipitates by filtration to prevent column clogging and concentration of matrix components above the column bed.

Load 25 mL of the diluted sample onto an immunoaffinity column OtaCLEAN to quantitatively bind the Ochratoxin A. Then wash the column with 10 mL of deionized water. Use the wash solution to rinse the template tube and then the OtaCLEAN column. Elute the column with 2 mL of methanol after short drying time. Make sure that the methanol incubates into the column bed for 5 min to ensure complete denaturation of the antibodies.

Find more details, recovery rates, HPLC-conditions and chromatograms here.

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