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Aflatoxins B/G and Ochratoxin A in Dates


The Date - "Gold of the Orient"

The „real date palm“ is a plant species from the palm family. It is an ancient oriental cultivated plant whose human use can be traced back to the year 6000 BC.

Dates are rich in sugar, have a high calorie content and grow mainly in warmer regions of the earth, so they are also known as „bread of the desert“. In dried fruit and especially in dried dates, aflatoxins B/G and ochratoxin A can be formed due to incorrect storage conditions and during the drying process. Since these toxins can be highly harmful to human and animal health, tests are carried out regularly. In order to test extracts (e. g. from dates), for both aflatoxins B/G and ochratoxin A in just one step, LCTech has developed the combined immunoaffinity column Afla-OtaCLEAN, which can be used in combination with the EluVac Vacuum Manifold, among others.

Parallel Clean-up - EluVac Vacuum Manifold

Aflatoxins B/G and ochratoxin A are the two most regulated mycotoxins and are formed by molds of the genus Aspergillus and Penicillum. Since mycotoxin clean-up is very important today, LCTech has developed the EluVac Vacuum Manifold as a way to efficiently clean-up more samples in combination with the Afla-OtaCLEAN immunoaffinity column and to speed up this way the clean-up process.

More samples in less time and flexibly adaptable. The Vacuum Manifold EluVac allows the simultaneous clean-up of up to 20 samples, which means that the sample throughput in your laboratory can be increased enormously with only little effort. The EluVac can also be easily and quickly adapted to other SPE - applications in environmental, food and feed analysis.

Processing Protocol

Homogenise 10 g dates with 1 g sodium chloride and extract through 50 mL methanol/water (80/20 (v/v)) and 25 mL of n-hexane to defat and remove essential oils. Run the extraction for at least 30 min. to achieve maximum extraction efficiency.

Subsequently, filtrate the raw extract and dilute 10.5 mL of the n-hexane free phase with 64.5 mL of PBS. In case of precipitation, filtrate the sample through a glass fibre filter to prevent clogging or coelution of matrix components from the column.
Load 50 mL of the diluted sample onto an immunoaffinity column Afla-OtaCLEAN to quantitatively bind the aflatoxins and the ochratoxin A. Wash the column with 10 mL of deionised water. Use the wash solution to first rinse the sample reservoir and then the Afla-OtaCLEAN column. Next step is to dry the column with a short stream of air.

Then elute the column with 2 mL of methanol. Make sure that the methanol incubates within the column bed for at least 5 min. to ensure complete denaturation of the antibodies.

Simplify the process of sample clean-up by using the EluVac Vacuum Manifold for manual processing. Process up to 20 columns in parallel at individual column flow rates and achieve a huge increase in your sample throughput.

Find more details, recovery rates, HPLC-conditions and chromatograms here.

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