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Ochratoxin A in Beer



Soon, it is time again and fans throughout Germany will be cheering on the matches of the Football World Cup 2018. Whether at large public viewings with huge LED screens or at a cosy barbecue in the own garden - one thing most people would not want to miss: a delicious cool beer. The variety is enormous, as there are currently around 7,500 types of beer. Beer is obtained by fermentation of substances containing starch. According to the German Purity Law, issued by Duke Wilhelm IV of Bavaria on 24 April 1516, only malt, hops, water and yeast is allowed for production. In 2016, the Purity Law became 500 years old, making it the oldest food law regulation in force today. 

However, beer is still a cereal product and therefore cannot always do justice to the term „pure“. The challenge is posed by toxic moulds that can develop during cultivation, storage, and processing of cereals leading to a loss in quality. For this reason, we have examined some types of beer for you this month. Chromatograms, recovery rates and a processing protocol are available on the following pages. 

Immunoaffinity Columns OtaCLEAN for Ochratoxin A

In order to preserve the good quality of beer, LCTech supports laboratories world­wide in the field of sample clean-up in food and feed analysis. Especially for the clean-up of ochratoxin A we offer the immunoaffinity columns OtaCLEAN, a solu­tion that convinces with very good recoveries even for difficult matrices. In addi­tion to the convenient 3 mL format, they are also available in a 1 mL format and are suitable for manual and automated processing, e.g. with the robotic system FREESTYLE SPE. 

Simply prepare the beer sample according to the processing protocol on the fol­lowing page, equip the racks with the samples, configure the desired method in the software and press START - while the system is processing sample by sample, you can use the time gained for other important tasks in the laboratory.

Manual Processing Protocol

Degas 20 mL of Beer by sonication. Add 8 mL of 3 % NaHCO3-solution and mix the sample material. Filtrate the raw extract and dilute 10 mL of it with 40 mL PBS. Load 50 mL (represents 7,14 g) of the sample onto a OtaCLEAN co­lumn. Wash the sample reservoir afterwards with 2 x 5 mL dei­onised water. 

Elute the toxin with 2 mL methanol of the column. Keep in mind that the column bed is incubated with methanol for 5 minutes in order to ensure a fully denaturation of the antibo­dies and release of toxins.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.

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