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Ochratoxin A in Rice


Cultivation and storage of food and animal feed may contribute to the spread of mold which produce mycotoxins - toxic secondary metabolites. Their consumption can lead to serious health damage in both humans and animals. Ochratoxin A is a naturally occurring mycotoxin, which is produced by various Aspergillus and Penicillium species as primary contaminant under bad storage conditions in various food and feed stuffs. It can be found in numerous types of grain like rice, dried fruits, or nuts.

Automated Sample Preparation via FREESTYLE SPE

Sample clean-up via SPE-column belongs to the protracted and monotonous routine tasks in any analytical laboratory - how-ever, this job demands highest concentration because the samples should not get mixed up. This is an ideal process step for automated processing.  The FREESTYLE SPE has unique possibilities for sample preparation using solid phase extraction (SPE).Diligently day and night, the longest running applications in mycotoxin analysis will be accurately processed. The FREESTYLE SPE can be used for all types of mycotoxins columns. Elution can be conveniently carried out in a 2 mL or 5 mL flask, such that the eluted volume can be adjusted quickly to a precise value.

Each already approved manual SPE method in the laboratory can be automated in a quick and easy manner. The application fields are wide: from mycotoxin and environmental analysis up to forensic applications and  doping control samples. 

The next generation of automated sample preparation. 
Flexibel, precise, modular.

Protocol of Processing

Preparatory Steps for the Following Processing
Extract 20 g homogenised rice with 100 mL methanol/water (80/20 (v/v)). The extraction should be performed 10 minutes. Filtrate the raw extract through a glass fiber filter (Whatman GF/A), in order to remove turbidities and precipitations.

Manual Processing
Dilute 12 mL sample with 48 mL PBS and load 25 mL of the sample (equates to 1 g matrix) onto the OtaCLEAN immunoaffinity column. Wash the column with 10 mL deionized water and dry it afterwards by flushing air through it.

Elute the toxin with 2 mL methanol. Keep in mind that the column bed is incubated with methanol for 5 minutes in order to ensure a fully denaturation of the antibodies.

Automated Processing via FREESTYLE SPE
For automated processing put the sample in the robotic system, parameterize the method with a few mouse clicks in the software and start the system. You can save the method in the system and call it up or you can adapt it anytime for other applications.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.

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