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Aflatoxins B/G in Biscuit Flour (Fish Feed)

Mycotoxins

Biscuit Flour

Biscuit flour is made of residues from baked goods. It is widely used in further processing as a component of feeder feed, which is used as an attractant in fishing. The floury consistency dissolves quickly in water, while the sweet biscuit flavour attracts the fish and keeps them at the fishing spot for a long time, as the flour is not filling. 

In the following we examined biscuit flour for aflatoxins B/G. The results obtained can also be transferred to ordinary baked goods such as biscuits or cakes. 

Mycotoxins in Food and Feed

Incorrect or prolonged storage can lead to the formation of mould toxins as metabolic products of various Aspergillus and Penicillium species, so-called aflatoxins, in the silos or bags in which biscuit flour is stored. In addition, mycotoxins can already be produced on the field during the cultivation of cereals, for example. The consumption of food and feed contaminated with mycotoxins can lead to serious health problems for humans and animals. In livestock breeding in particular, mycotoxin contamination in feed can result in high economic losses. 

Fast and Efficient Clean-up with AflaCLEAN Select

LCTech supports laboratories wordwide in the clean-up of mycotoxins in food and feed analysis in order to preserve good quality. Particulary for the clean-up of Aflatoxins B1, B2, G1 and G2 we offer AflaCLEAN Select, an immunoaffinity column for high sample throughput with excellent performance and low price. Under cool coonditions between 4 and 8 °C the column has a shelf life of 9 months.

The convenient 3 mL polypropylene format is suitable for manual as well as for automated processing, e. g. with the robotic system FREESTYLE SPE. Diligently day and night, the system processes reliable various applications in mycotoxin analysis. 

Processing Protocol

Homogenise 20 g of biscuit flour and add 2 g of sodium chloride. Extract the mix with 100 mL of methanol/water (80/20 (v/v)). In order to remove fat, add 50 mL of n-hexane during the extraction. Perform the extraction for at least 10 minutes to nsure high extraction efficiencies.

Filtrate the raw extract and dilute 10.5 mL with 64.5 mL PBS. Filtrate the sample again through a glass fiber filter in order to remove precipitations. Load 50 mL of sample (represents 1.4 g matrix) onto an AflaCLEAN Select column. Wash the column afterwards with 2 x 5 mL de-ionised water. 

Dry the column with a short flush of air and elute the aflatoxins with 2 mL of methanol. Keep in mind that the column bed is incubated with methanol for 5 minutes in order to ensure a fully denaturation of the antibodies.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.

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