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Aflatoxins B/G and Ochratoxin A in puffed Millet


Millet belongs to the family of sweet grass (Poaceae) and is mainly cultivated in tropical and subtropical areas. There are over 600 million types of millet worldwide. Thereby, depending on the type, the color varies between white-gray, yellow and red-brown. The tiny, small-sized millet seeds provide a very high nutrient density, are completely gluten-free, and a wonderful ingredient for a fast cooking. In many parts of Africa and Asia, millet is one of the most important basic foodstuffs. 

Meanwhile, millet is also cultivated in many European countries, including Germany. When importing, however, our quality requirements are not always fulfilled. This is shown by the border controls of the EU where aflatoxins and ochratoxin A repeatedly lead to rejection.

Combined Immunoaffinity Column Afla-OtaCLEAN for Clean-up of Mycotoxins

One for all- fast and efficient

Aflatoxins and Ochratoxin A are naturally occurring mycotoxins and are produced by funghi in wet or rather wrong storage. They are often found together in many foods and feeds. In order to reduce the workload and to halve the working time, a way of testing several mycotoxins in one single operation was needed. For this reason, LCTech developed the combined immunoaffinity column Afla-OtaCLEAN for the clean-up of aflatoxin B1, B2, G1, G2 and ochratoxin A. Since LCTech produces both antibodies and clean-up columns, comprehensive quality testing throughout the entire manufacturing process ensures high product quality.

All clean-up columns are suitable for automated processing and are dedicated for use in the LCTech devices FREESTYLE SPE or FREESTYLE ThermELUTEā„¢. Time-consuming and tedious tasks are not a problem for the FREESTYLE system. These tasks are processed reliably and consistently during day, night and over the weekend. Beside the LCTech immunoaffinity and SPE columns the FREESTYLE SPE can be used for all types of mycotoxin columns of leading manufacturers.

Protocol of Processing

Homogenise 10 g of puffed millet and add 2 g sodium chloride, 100 mL methanol/water (80/20 (v/v)) and 50 mL n-hexane to remove fat and oil. The extraction should be performed for 10 - 15 minutes. 

Filtrate the raw extract and dilute 2 mL with 12 mL PBS (contains 8 % Tween). Load the sample completely onto an immunoaffinity column Afla-OtaCLEAN. Afterwards wash the column with 2 x 5 mL deionised water and load this solution onto the IAC-column, too. 

Dry the column and elute the toxin with 2 mL methanol. Keep in mind, that the methanol incubates for 5 minutes into the column bed, in order to dissolve the antibody toxin bond completely. Dilute the sample to HPLC conditions and measure it afterwards. 

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.

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