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Mycotoxins in Millet


The Millet

Millet is a collective name for small-fruited spelt cereals with 10 - 12 genera belonging to the sweet gras family. Already 8,000 years ago, millet was used to make dishes like unleavened flatbread. In Africa, millet is one of the staple foods along with rice, maize and wheat.

In addition to producing food, the plant is used today as building material, for paper production and for brewing beer. The plant is considered to be very rich in minerals: Besides fluorine, sulphur, phosphor, magnesium and potassium, millet contains particularly high levels of silicon (silicic acid), iron and vitamin B6 compared to other cereals.

Incorrect storage conditions can cause certain mycotoxins to develop in the grain. Field fungi already produce mycotoxins during growth and fruit rispening. Since these are highly harmful to human and animal health in excessive quantities, tests are regularly carried out on cereals like millet.

All in One - From Aflatoxin to Zearalenone with CrossTOX®

Mycotoxins are natural, secondary metabolic products of various moulds with toxic effects. Since the clean-up of mycotoxins is of great importance today and therefore it is a good idea to test extracts for several mycotoxins in just one step, LCTech has developed the multi-mycotoxin clean-up column CrossTOX®. This column can be used for manual as well as automated processing with the FREESTYLE SPE robotic system.

LCTech`s CrossTOX® columns enable highly efficient sample clean-up of regulated and expected mycotoxins. At the same time, they improve the conventional dilute-and-shoot application by a QuECHERS-like process. With the CrossTOX® column you are able to clean-up a wide range of different matrices. 

Processing Protocol

Homogenise 10 g of millet and exract the sample through 50 mL acetonitrile/water (84/15 (v/v) + 1 % acetic acid). Run the extraction for 5 to 10 minutes, depending on the device used, to achieve high extraction efficiency.

Subsequently, filtrate the raw extract or alternatively clarify it by centrifugation at 3000 x g for 5 minutes. Continue to use the resulting clear supernatant.

Load maximum 3 mL of the sample volume (for different matrices a maximum of 0.5 to 1 mL) onto a CrossTOX® column at a constant flow rate of 1 - 2 mL/min. Collect the flow through in a sample vial for analysis by LC-MS/MS.

Find more details, recovery rates and UPLC-conditions here.

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