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Aflatoxins B/G and Ochratoxin A in Oat Cakes

MYCOTOXINS

Oat Cake

Oat cakes are a popular sweetness worldwide and are available in many different varieties: with chocolate chips, with flaked almonds and many more creations. The basis of oat cakes are, as the name already suggests, oat or oat flakes. The name „oat“refers to the spelt or husked grain.

Inside the grain you will find the core. For the production of flakes, the oat core is humidified and then rolled out by a flaking roller. Only after the drying process, the typical oat flakes are ready to use. The main growing areas are Ireland, Germany and Scotland. Other growing areas are also located in North America and West Asia.

One for All - Combined Immunoaffinity Column Afla-OtaCLEAN

Aflatoxins B/G and ochratoxin A are produced by fungi in wet storage and are often found together in many food and feed products, such as oat cakes. LCTech offers the perfect solution to save you time during the clean-up process.  Analyse your sample for several mycotoxins in only one step. LCTech‘s combined immunoaffinity column Afla-OtaCLEAN makes it possible. The column is suitable for manual processing but also for automated clean-up with the robotic system FREESTYLE SPE.  

You can also combine the practical SMART columns from LCTech. Plug an AflaCLEAN SMART column and  OtaCLEAN SMART column on top of each other and start the manual clean-up of aflatoxins B/G and orchatoxin A at once.

Processing Protocol

Homogenise 20 g of oat cakes with 2 g of sodium chloride. Extract the sample with 100 mL methanol/water (80/20/ (v/v)) and 50 mL of n-hexane in order to remove fat and oil. For high extraction effiecincies, continue the extraction (depending on extraction device) for at least 30 minutes. 

Filtrate the raw extract and dilute 7 mL of the n-hexane free phase with 43 mL PBS. For a better phase separation between the methanolic phase and the n-hexane phase, centrifuge the extract for 5 minutes at 3000 x g. The reduction of the methanol content may cause turbidity in the diluted sample. Therefore, filter the sample again to avoid clogging of the column. 

Load 50 mL of the sample (correspond to 1.4 g matrix equivalents) onto an Afla-OtaCLEAN immunoaffinity column. Wash the vial with 5 mL deionised water. Load the rinsing solution onto the column. Wash the column again with 5 mL deionised water. Then remove any remaining liquid from the column by flushing air through it. Elute the column with 2 mL methanol. Ensure that the methanol is allowed to act on the column bed for 5 minutes to ensure a fully denaturation of the antibodies and release of toxins.

Find more details, recovery rates, HPLC-conditions and chromatograms here.

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