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Aflatoxins B/G und Ochratoxin A in Turkey Rearing Feed

Mycotoxins

Turkey Meat

Goulash, skewer or steak - turkey meat is one of the most popular meat in Germany. It is not only versatile and easy to prepare, but also a supplier of valued protein, essential iron, B vitamins and minerals. Further, it contains a high proportion of niacin and thus stimulates the metabolism. Furthermore, turkey meat is low in fat and calories.

To ensure the good quality of our meat and thus animal health, the feed, as  in this case turkey rearing feed, plays a decisive role. Mycotoxins in animal feed can lead to significant losses in yield and quality of agriculture, as they have a major influence on animal health. Feeding composition could contain mycotoxins by cereal dust or low quality ingredients.

Automated Sample Preparation with FREESTYLE SPE

Since 1998, LCTech has been developing and supplying products and methods for the preparation and analysis of food, aninmal feed and environmental samples. Thereby we can continue to consume our food with a clear conscience.

The product range includes semi- and fully automated sample preparation systems as well as consumables for the analysis of contaminants and residues. The automated robotic system FREESTYLE SPE offers unique possibilities for sample preparation by solid phase extraction (SPE)

Any manual SPE method that has already proven itself in the laboratory can be automated quickly and easily. Simply carry out the preparatory processing steps as described on the following page. Then position the sample in the FREESTYLE SPE, parameterise the method in the software with a few mouse clicks and start the system - done.

Processing Protocol

Homogenise 10 g of matrix (turkey rearing feed) and add 1 g of sodium chloride. Extract the sample through 50 mL of methanol/water (80/20 (v/v)) and add 25 mL n-hexane in order to remove fat and essential oils. For high extraction efficiencies, continue the extraction for 10 minutes. 

Filtrate the raw extract and dilute 10 mL of it with 64.5 mL of PBS. In case of precipitations, filtrate the sample again.

Load 50 mL of the sample (corresponding to 1.4 g matrix) onto an Afla-OtaCLEAN column for clean-up of both toxins at once or individually depending on the toxin onto an AflaCLEAN or OtaCLEAN column. Wash the column with 10 mL of deionised water each and dry it afterwards by flushing air through it. 

Elute the toxin with 2 mL of methanol. The column bed is incubated with methanol for 5 minutes to ensure a fully denaturation of the antibodies and release of toxins.

Dilute the sample to eluent conditions and measure it afterwards via HPLC with fluorescence detection or LC-MS.

Find more details, recovery rates, HPLC-conditions and chromatograms here.

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