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Aflatoxins B/G in Corn

Mycotoxins

Fully Automated Sample Preparation

Aflatoxins in Corn

Aflatoxin B1 is the most dangerous mycotoxin to human among all aflatoxins. As consequence to repeating exceedance of the maximum content of aflatoxin B1 in corn samples from different countries, the ad-hoc monitoring plan has been implemented in 2012. It divides the countries of origin in different categories (high, medium, low risk). Every QS-certificated company, which obtains corn or corn-processing products, must strictly follow the directives given. [Source: Ad-hoc monitoring plan for aflatoxin B1 in corn, QS Quality and Safety GmbH, Bonn]

Automated Sample Preparation around the Clock

Precise, simple and fast with LCTech products

LCTech supports you in your daily laboratory routine with a whole range of reliable and high quality products at favorable prices: from immunoaffinity columns and devices for derivatisation up to complete robotic systems for fully automation in mycotoxin analysis.

Our systems processes your samples by day, night and even at weekends - reliable and unattended. Each manual method, already proved at your laboratory, can be transferred to automation on the robotic system FREESTYLE SPE. That gives you more time for other important activities.

On the following page we compare the fully automated processing via FREESTYLE SPE and the manual processing with a protocol, recovery rates and a chromatogram.

Processing Protocol 


Preparatory Steps for the Following Processing
Extract 20 g homogenised corn with 100 mL methanol/water (80/20 (v/v)). The extraction should be performed for 10 minutes. Filtrate the raw extract trough a glass fibre filter (Whatman GF/A), in order to remove turbidities and precipitations.

Manual Processing
Dilute 7 mL sample with 43 mL PBS and load 25 mL sample onto the immunoaffinity column AflaCLEAN. Wash the column with 10 mL deionized water and dry it afterwards by flushing air through it. Elute the toxin with 2 mL methanol. Keep in mind that the column bed is incubated with methanol for 5 minutes in order to ensure a fully denaturation of the antibodies and release of toxins.

Automated Processing with FREESTYLE SPE
For automated processing put the sample in the FREESTYLE SPE, parameterize the method with a few mouse clicks in the software and start the system. You can save the method in the system or you can adapt it anytime for other applications.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.

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