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Aflatoxins B/G in Chestnuts

Mycotoxins

FREESTYLE SPE for Automated Sample Preparation

Sample Preparation and Analysis

Automated sample preparation has never been as easy as it is when using the FREESTYLE SPE robotic system. You can handle your SPE-processes fully automated around the clock and even at the weekend.

Flexible in sample loading and elution, the system can be used in a wide area of applications - from the mycotoxin analysis to forensics field. Transfer your manual method to the FREESTYLE SPE. And you have more time for other important activities.

Processing protocol -manual / automated-

Homogenize 20 g of chestnuts and add 2 g sodium chloride. Extract the sample material with 100 mL (methanol/water (80/20 (v/v))) and 50 mL n-hexane to remove fat and essential oils. The extration should be conducted for at least 10 minutes.

Filtrate the raw extract and dilute 10.5 mL with 64.5 mL PBS. Load 50 mL (represents thereof 1.4 g) onto the AflaCLEAN immunoaffinity column and wash the column with 10 mL de-ionised water.
For automated processing with the robotic system FREESTYLE SPE, parametrize a maximum flow rate of 2 mL/min for sample injection and washing of the AflaCLEAN columns

Dry the column by flushing air through it. For automated processing two possibilities are available: either the syringe pump circulates 40 mL of air by 20 mL/min or the optional nitrogen-port dries the column with nitrogen. 

Elute the toxins with 2 mL methanol. Keep in mind that the column bed is incubated with methanol for at least 5 minutes in order to ensure the complete denaturation of the antibody.
When using automated processing via FREESTYLE SPE, choose a slow flow rate (0.2 – 0.5 mL/min) to ensure the elution efficiency. 

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.  

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