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Aflatoxins B/G in Peanut Butter


From Peanuts to Peanut Butter

The peanut is a legume and grows underground. The fruit prefers tropical and subtropical climates, so the typical growing areas are the USA, Argentina, Brazil, Egypt and Senegal. Peanuts contain a lot of vegetable protein, many unsaturated fatty acids and other important nutrients.

Moreover, they are not only delicious and nutritious, but also indispensable in the food and feed industry. To make peanut butter, vegetable oil, salt and sugar are added to the peanuts and processed into a homogeneous spreadable mass. This is how you get the energy-rich spread.

Small + Fast + Economical = SMART

Strict EU-wide legal regulations for the maximum content of mycotoxins and constant controls of our food and feed are essential, because the consumption of too high levels of contamination can lead to serious damage to human and animal health.The challenge many laboratories have to face nowadays is to be able to process many samples as quickly as possible. To make this task easier, LCTech has developed the 3 cm AflaCLEAN SMART columns in addition to the 3 mL AflaCLEAN immunoaffinity columns. The SMART columns not only impress with their small size, but also save you money.

1/5 Time, 1/5 Solvent and 1/5 Waste = 100 % Performance

Processing Protocol

Homogenise 20 g of peanut butter and add 2 g of sodium chloride and extract through 100 mL methanol/water (80/20 (v/v)) and 50 mL of n-hexane to remove oils. For high extraction efficiencies, continue the extraction for at least 10 - 20 minutes. Filtrate the raw extract and dilute 7 mL with 43 mL PBS.

With AflaCLEAN column
Load 50 mL of sample (represents 1.4 g Matrix)  onto the AflaCLEAN immunoaffinity column. Wash the column with 10 mL water. Dry the column by flushing air through it. Elute the toxins with 10 mL methanol.

With AflaCLEAN SMART column
Load 10 mL of sample onto the immunoaffinity AflaCLEAN SMART column. Wash the column with 2 mL water  at a flow rate of 3 mL/min. Dry the column by flushing air through it. Elute the toxins with 400 ┬ÁL methanol. 

Ensure that the methanol incubates in the column bed for 5 minutes to completly denaturate the antibodies and release the toxins.

Find more details, recovery rates, HPLC-conditions and chromatograms here.

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