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Aflatoxins B/G in Peanuts with Shell


FREESTYLE ThermELUTE™: ppt instead of ppb

Sample Preparation and Analysis

The combination of the FREESTYLE ThermELUTE™ and an HPLC unit with FLD enables the fully automated analysis from extract to chromatogram of the aflatoxins B1, B2, G1, G2 and M1 as well as Ochratoxin A. Be it food or feed, the FREESTYLE ThermELUTE™ is the only fully automated processing equipment that can analyse mycotoxins using immunoaffinity columns, thermal elution and subsequent HPLC. And it‘s so easy in daily routine:

Processing protocol with FREESTYLE ThermELUTE™

Homogenize 20 g of peanuts and add 2 g sodium chloride. Extract the sample material with 100 mL methanol/water (80/20 (v/v)) and 50 mL n-hexane to remove fat and oils. The extraction should be conducted for at least 5 to10 minutes.
Filtrate the raw extract and dilute 7 mL with 43 mL PBS.

Next place the sample as well as the immunoaffinity column AflaCLEAN SMART into the robotic system FREESTYLE ThermELUTE™. Choose the required method from the FREESTYLE software and start the system. From now on the FREESTYLE ThermELUTE™ takes over the further processing of your sample.
Up to 10 mL of the sample can be cleaned-up by the AflaCLEAN SMART column. The chromatograms on the following page show a sample load of 5 mL, 2.5 mL and 1 mL sample.
The column is washed with 2 mL of deionized water and is eluted via the ThermELUTE™-technology. The eluate is transferred directly and quantitatively as sample loop partial filling into the injection system of the HPLC unit.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.  

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