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Aflatoxins B/G in Brazil Nut

Mycotoxins

Fully Automated Sample Preparation

Brazil Nuts: A Must in Winter Season

Especially in the months October till December nuts are very popular. Brazil nuts have a very strongly aromatic taste and are full of positive ingredients like zinc, iron or vitamin E. Aflatoxins often damage nuts due to incorrect or too long storage. Aflatoxins are toxins, which are not immediately recogniseable by smell, taste or with the naked eye. Particulary for the import from third countries the implementing regulation of the European commision, (EG) Nr. 1152/2009 set intensive sampling and investigations in order to control the  high risk of aflatoxin contamination. 

Mycotoxin Analysis via FREESTYLE ThermELUTE™

The robotic system FREESTYLE ThermELUTE™ facilitates fully automated mycotoxin analysis in combination with any HPLC system. It achieves such sensitive results that the user can be sure to measure below the European limits. With the unique technique of thermal denaturation the comprehensive automation „from raw extract to chromatogram“ is realised without any manual working steps. 

The system achieves the high sample throughput of 500 samples/week by using the SMART immunoaffinity columns with a size of only 3.5 cm. Through miniaturisation of the overall process, not only the processing time of the sample is dramatically reduced, but also the required amounts of sample and solvent. Each sample is processed with a SMART column, which grants top performance for each sample and reliably prevents cross-contamination.

... easy and reliable sample preparation around the clock!

Protocol of Manual Processing

Homogenise 10 g of brazil nuts and add 2 g of sodium chloride. Extract the sample with 100 mL (80/20 (methanol/water (v/v))) and dilute with 50 mL n-hexane in order to remove fat and oils. Filtrate the sample and dilute 2 mL with 12 mL PBS buffer (pH value 7.2) which contains 8% Tween20. Depending to the detector sensitivity you can dilute the extract further (in this case 1:4).

FREESTYLE ThermELUTE™ loads the extracted, diluted and filtrated sample automatically onto the high-performance AflaCLEAN SMART column. Due to the high sensitivity of the FREESTYLE ThermELUTE™ the amount of matrix can be either drastically reduced (down to 0.02 g) or it can be achieved a very high measure sensitivity until the range of ppt.

With a applied flow rate of 1.5 mL the column will be washed with 2 mL deionized water. Afterwards the column will be thermal eluted by ThermELUTE™ technology and on-line injected directly into the HPLC. The results are excellent, high-resolution chromatograms within 10 minutes measuring time.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.

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