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Deoxynivalenol in Grain



Deoxynivalenol (DON) is a worldwide naturally occurring mycotoxin, which is toxic for humans and animals. The toxin is produced by fungi of the fusarium genus e.g. fusarium culmorum and fusarium graminearum, which are particularly abundant in various cereal crops (wheat, barley, oats).
In agriculture, especially livestock farming, DON impact is as an economic factor, as the toxin causes considerable productivity losses due to deterioration in animal health. For example, a weight gain decrease by 26 % was observed in pigs that ingested DON through their feed. 

SPE Clean-Up Columns for Analysis of Deoxynivalenol

This toxin is most analysed with an HPLC / UV detector or LC/MS, especially for confirmation analysis. In all processes, thorough sample preparation increases the life time of the analytical system and also the life time of the HPLC system. In addition, pre-cleaning reduces interferences by matrix removal and nearly halves the chromatography time.

For clean-up of deoxynivalenol in food and feed samples, LCTech developed the SPE columns DONeX. They exclude above described interferences through matrix and associated long chromatographies. The DONeX columns are suitable for many common matrices such as corn, barley, oats, wheat, rye, cereal-based feed, but also for more complex matrices such as müsli, pasta or breads.
On the following pages, we examined some grain products for you.

The clean-up column is available in a 3 mL format and is thus suitable for manual as well as for automated processing around the clock with the LCTech robotic system FREESTYLE SPE.

Processing Protocol

Homogenise 10 g of durum wheat/wheat flour/chicken feed and extract it with 50 mL acetonitrile/water (84/16 (v/v)) in a beaker at high-speed level, e.g. with an Ultraturrax. Filtrate the extract with a folded filter.

Load 20 mL (represents 4 g matrix) using a vacuum manifold (e.g. LCTech EluVac) onto the DONeX column. Wash the column with 10 mL of acetonitrile/water (84/16 (v/v)). The flowthrough- and the washing-fraction are combined.

Evaporate 7.5 mL of the mixed solution (represents 1 g matrix) with nitrogen until dryness and dissolve it afterwards in 0.5 mL of HPLC-solvent.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.

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