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Ochratoxin A in Beer

Mycotoxins

Sample Preparation - Manual and Automated

The International Beer Day

The international beer day is a celebration on the first Friday of every August, this year it´s the 5th of August. On this occasion our laboratory has tested beer as matrix of the month for you.

We are happy to provide you below chromatograms, recovery rates and a protocol for the manual processing. 

The challenge many laboratories have to face nowadays is to be able to process many samples as quickly as possible. To facilitate this task, LCTech has developed the OtaCLEAN™ SMART immunoaffinity column for the analysis of ochratoxin A in food. Ochratoxin A is a naturally occuring mycotoxin which is produced by various Aspergillus and Penicillium species as primary contaminant in various food and feed stuffs.

The Robotik System FREESTYLE ThermELUTE™

Fully Automated Mycotoxin Analysis - Twenty-for-seven

Using OtaCLEAN™ SMART your automated processing is especially effective. The best of all: Your sample throughput rises up to 500 samples per week and your solvent consumption is clearly reduced. The robotic system FREESTYLE ThermELUTE™ takes over your time consuming work and processes the SMART columns fully automatically: from raw extract to chromatogram. 

Extract, filtrate and dilute your samples in accordance with the processing protocol, put them into the FREESTYLE ThermELUTE™, equip the racks with the SMART columns, configurate your method in the software and start the FREESTYLE ThermELUTE™. From now on the robotic system does the work for you.

Protocol of Manual Processing

Degas 20 mL of beer by treatment with ultrasound. Add 8 mL of (3%) NaHCO3-solution and mix the sample material. In order to remove precipitations, filtrate the sample with a glass filter. Afterwards add 12 mL PBS to 3 mL of the material and mix the sample. 

Load 10 mL of the filtrated sample onto the immunoaffinity column OtaCLEAN SMART and rinse the sample reservoir then with 2 mL of water. Load the rinsing solution onto the column, too.  If there is foam at the surface of the column, wash it again with 2 mL of water. Dry the column by flushing air through it subsequently.

Eluate the toxin through 400 µL methanol. Take care, that the methanol incubates 5 minutes into the column bed, to ensure a full denaturation of the antibodies. Adjust the eluent afterwards to fluidic contitions of the HPLC or inject the sample directly into the HPLC.

Find more Details, Recovery Rates, HPLC-Conditions and Chromatograms here.  

Back to: Matrix of the Month

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