Sample Preparation and Analysis
Updating Regulation on Imports of Food and Feed
2016 the regulations (EG) nr. 669/2009 and (EU) nr. 884/2014 have been updated. This regulations stipulate, that peanut products from brazil, nutmeg and paprika (capsicum annuum) from india and indonesia must be subject to stricter controls concerning aflatoxins.
On this occasion our laboratory provides a fast and efficient extraction protocol with recovery rates and chromatograms for the analysis of aflatoxins (B/G) in paprika.
Fast and Efficient Sample Clean-up
Immunoaffinity Columns AflaCLEAN for Aflatoxins B/G
The immunoaffinity columns AflaCLEAN of LCTech optimise sample preparation in the laboratory. The columns possess a very high matrix tolerance and are able to bind aflatoxins highly specific. With only three provided extraction protocols, all matrices from A to Z, can be tested whilst obtaining excellent recovery rates, paprika too.
AflaCLEAN columns are suitable for sample preparation in aflatoxin analysis using HPLC with fluorescence detection or LC-MS. They are designed for clean-up of aflatoxins B1, B2, G1 and G2 in food and feed.
The AflaCLEAN columns of LCTech are suitable for both, manual and automated processing e. g. with the robotic system FREESTYLE SPE. This system enables an automated processing of samples whilst obtaining excellent reproducibility and a high sample throughput around the clock, even at weekends!
Beside the analysis of mycotoxins, FREESTYLE SPE can be also used for doping control, H53-analysis or soil samples.
Protocol of Manual Processing
Homogenise 10 g of paprika (capsicum annuum) and add 1 g of natrium chlorid. Extract the sample material with 50 mL methanol/water (80/20 (v/v)) to remove fat and essential oils. The extraction should be conducted for at least 20 minutes.
Filtrate the raw extract and dilute 2 mL with 12 mL PBS (contains 8% tween). Load 14 mL of the matrix onto the immunoaffinity column AflaCLEAN™ (for aflatoxins B/G) and wash the column with 10 mL deionised water.
Dry the column. Afterwards elute the toxin with 2 mL methanol. Keep in mind that the column bed is incubated with methanol for at least 5 minutes in order to ensure the complete denaturation of the antibody.