Immunoaffinity Columns for Clean-up of Aflatoxin M1
The AflaCLEAN M1 Select columns can be processed either manual or automated, for example, with the LCTech robotic system FREESTYLE SPE. The loading capacity of the columns is max. 100 ng Aflatoxin M1.
The immunoaffinity column AflaCLEAN M1 Select was developed for the sample preparation within the food analysis via HPLC with fluorescence detection and LC-MS, respectively.
Advantages at a Glance
- 3 mL format
- Shelf life: 12 months cooled at 4 to 8 °C
- Loading capacity: 100 ng Aflatoxin M1
- Recovery rates: Aflatoxin M1 > 90 %
- Suitable for automated processing
Functionality of the Columns
The monoclonal antibody is specifically designed for this application and thus guarantees best results, even with difficult matrices. The working mode is based on the principles of immunoaffinity. The material within the columns is covered with antibodies which are directed against aflatoxin M1. Upon application of an extract onto the column, the aflatoxins are retained, whereas the remaining matrix components pass through the column. After a washing step removing matrix interferences, the aflatoxins can be eluted quantitatively from the column with methanol and subsequently measured with HPLC.
Quality is important to us!
Since the antibodies as well as the immunoaffinity columns are produced by LCTech, numerous in-house quality tests over the whole production process guarantee a persistent quality. LCTech meets the high demands of European and international legal requirements concerning mycotoxin analysis and controls every single production step. A detailed quality certificate is included in each pack.
In the following recovery rates (%) of aflatoxin M1 in different matrices are shown, cleaned-up with
LCTech´s 3 mL immunoaffinity columns AflaCLEAN M1 Select and analysed with an HPLC/FLD system. The samples were spiked with 80 ppb aflatoxin M1 respectively. The results correspond to the performance specifications of EC 401 / 2006 (Section 4.3.1):
In the following some exemplary chromatograms are shown, which were obtained by using AflaCLEAN M1 Select columns: