Alfatoxin B/G and Ochratoxin A in Cumin

Mycotoxins

Cumin

Cumin – a wonder herb?! Cumin is mainly found in China, India and South America. It is more taste-intensive as the caraway, which is mostly used for cooking in European countries. Both species grow on parallel branches of the umbellifer. 

The high content of essential oils also helps to improve digestion and trigger a growth-inhibiting effect on bacteria and fungi. To digest indigestible foods, it used to be common to eat caraway seeds with a little sugar after a heavy, fatty meal. 

In order to preserve cumin, it is imported into Europe in dried form from the growing regions. During this drying process or under incorrect storage conditions, mycotoxins can be formed, which can reduce the quality of the spice and cause damage to human health. For this reason, there are strict EU-wide controls on imports from third countries.

The Immunoaffinity Column Afla-OtaCLEAN for Clean-up of Mycotoxins

Clean-up of difficult matrices, such as coffee or spices, presents many challenges in everyday laboratory work and cost a lot of time. To facilitate these steps, LCTech developed the immunoaffinity column Alfa-OtaCLEAN. With the combined column, you can clean-up Alflatoxin B/G and Ochratoxin A simultaneously in all matrices, as well as cumin, quickly and with good recovery rates. 

The mycotoxins Alfatoxin B/G and Ochratoxin A are often found combined in the food and feed sector. With the Afla-OtaCLEAN column, you can clean-up both mycotoxins in only one step. This saves you half the money an half of the time.

Processing Protocol

Homogenise 10 g of cumin and add 2 g of sodium chloride. Extract the mixture with 100 mL methanol/water (80/20 (v/v)) and add 50 mL n-hexane in order to remove fat an essential oils. To ensure high extraction efficiencies, continue the extraction for at least 20 minutes.

Centrifugation may help to separate n-hexane phase as top layer from the methanolic bottom layer. Filtrate the raw extract and dilute 2 mL with 12 mL PBS (contains 8 % Tween20). In the next step load sample (corresponds to 0.2 g matrix) onto the immunoaffinity column Alfa-OtaCLEAN. 

Subsequently, dry the column with a short airflow. Elute the column with 2 mL of methanol. Keep in mind that the column bed is incubated with methanol for 5 minutes in order to ensure a fully denaturation of the antibodies and release of toxin. 

Find more details, recovery rates, HPLC-conditions and chromatograms here.

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