UV Derivatization Module for the Analysis of Aflatoxins
UVETM
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Increase of flourescence of Aflatoxin B1 and G1 by UV light |
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Comparable to Cobra cell, but no reagents needed |
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Extremely low-priced |
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Robust for every-day work
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The UVE derivatization module allows easily analyzing aflatoxins with HPLC. The reaction is a simple derivatization of B1 to B2a
The application can be done easily. Simply place the UVE between the HPLC and the fluorescence detector, switch on the system – ready:
Up to now reagents had to be added for the derivatization – whereas the photochemical reactor directly uses the HPLC eluant as reagent. The HPLC system remains clean and can immediately be used for other methods. Any tedious and long lasting rinsing of the system is no longer necessary.
A confirmation analysis of the aflatoxins B1 and G1 can be performed by switching off the system.
The system is very robust and long-living. It has an average shining duration of 8.000 hours.
Application notes for direct Download (pdf):
References
Muscarella, M. et al., Food Additives and Contaminants, Vol. 26, No. 10,
October 2009, 1402-1410, Validation of a confirmatory analytical method
for the determination of aflatoxins B1, B2, G1 and G2 in foods and
feed materials by HPLC with on-line photochemical derivatization and
fluorescence detection
Papadopoulou-Bouraoui A., Stroka J., Anklam E., J., AOAC Int. Vol. 85, No. 2,
2002, 411-416, Comparison of two post-column derivatization systems,
ultraviolet irradiation and electrochemical determination, for the liquid
chromatographic determination of aflatoxins in food
FAPAS Proficiency Test 04148 Report, Aflatoxins B & G in Maize,
October - November 2009
FAPAS Proficiency Test 04143 Report, Aflatoxins Analysis in Baby Food,
July - August 2009
Barricelli M, Kupfer R, Börner B, Deutsche Lebensmittel-Rundschau Spezial, Validated Method for Simultaneous Determination of Aflatoxins and Ochratoxin A in Paprika and Chilli Powder , September 2010
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